Because the identification afterward of the specific cytosolic components required for fusion is often difficult, we adopted instead a reverse approach in which individual components were added to the fusion reaction until the minimal requirements to reproduce vesicular fusion were identified

Because the identification afterward of the specific cytosolic components required for fusion is often difficult, we adopted instead a reverse approach in which individual components were added to the fusion…

Continue ReadingBecause the identification afterward of the specific cytosolic components required for fusion is often difficult, we adopted instead a reverse approach in which individual components were added to the fusion reaction until the minimal requirements to reproduce vesicular fusion were identified

Cell components were prepared using passive lysis buffer (E1910; Promega, Madison, WI, USA)

Cell components were prepared using passive lysis buffer (E1910; Promega, Madison, WI, USA). the adjacent PRBS. Luciferase assays indicated the DMR and distal PRBS constitute a novel RANKL distal regulatory…

Continue ReadingCell components were prepared using passive lysis buffer (E1910; Promega, Madison, WI, USA)