All point mutations were generated by following a manufacturer’s instructions (QuikChange II, Agilent)
All point mutations were generated by following a manufacturer's instructions (QuikChange II, Agilent). Protein expression and purification GST-GRK2RH, GST-p63RhoGEFDH/PHext, GST-TrioDH/PHext, GST-kalirinDH/PHext, GST-GAIP, and GST-RGS2 were expressed in BL21(DE3) transformed with…